ABSTRACT
Objective To determine the proper storage environment by comparing the activity of adipose-derived stem cell (ADSC) at 10％ PRP,10％ HS and 0.9％ NaCl.Methods ADSC was isolated and cultured.Then the ADSC was stored in 10％ PRP,10％ HS,and 0.9％ Nacl at room temperature (RT,about 26 ℃C) for 2 hours.After that the proliferation ability,survival rate and differentiation ability of the cells were detected.Finally storage media of ADSC was determined.Results Compared with 0.9％ NaCl,ADSC showed a better proliferation and higher differentiation ability at 10％ PRP and 10％ HS;besides,the survival rates of two groups were higher than the group of 0.9％Nacl.Among these groups,the survive rate of 10％ HS and 10％ PRP were 78.08％ and 75.68％ respectively,while it was 62.34％ in 0.9％ NaCl group.Conclusions 10％ HS is more suitable for short-term preservation of ADSC.
ABSTRACT
Objective To observe the effect of varying intensities of water-jet force on autologous fat graft viability.Methods Lipoaspirate was taken from 12 female patients undergoing waterjet assisted abdominal liposuction at our department.According to the intensity of water-jet force,the experimental group was divided into four subgroups:R1 (pressure,30 bar),R2 (pressure,50 bar),R3 (pressure,70 bar) and R4 (pressure,90 bar).Hand-held suction was taken as the control group C.Adipose tissue was filtered with cotton cushion and centrifuged at low speed,and the composition ratio of water and fat tissue from each group was observed.Calcein-AM/Hoechst 33342 staining was used to detect the viability of adipocytes.Results Fat aspirates was divided into four layers:oil layer,pure fat tissue,liquid and bottom sediment.Oil ratios of R1,R2,R3,R4 and C were (8.9 ± 2.3) ％,(9.6±2.1)％,(10.3±1.3)％,(14.2±1.6)％ and (9.5±1.8)％,respectively.There was no statistically significant difference between R1,R2,R3 and C (P＞0.05).Statistically significant difference was found between R4 and other groups (P＜0.001).Viability of adipocytes from R1,R2,R3,R4 and C groups were (88.1±2.8)％,(89.9±1.9)％,(84.8±2.3)％,(78.0±1.7)％ and (91.1±2.9)％ respectively.There was no statistically significant difference between R1,R2 and C (P＞ 0.05).Statistically significant difference was found between R3,R4 and C (P ＜ 0.05).Conclusions Viability of fat graft harvested under lower intensity of water-jet force (R1,R2) is higher than that harvested under higher intensity of water-jet force (R3,R4).